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exonuclease i enzyme  (New England Biolabs)


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    Structured Review

    New England Biolabs exonuclease i enzyme
    Exonuclease I Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 4852 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/exonuclease+enzyme/Exonuclease+I/bio_rxiv__64898__2026__02__23__707224-388-6-9
    Average 99 stars, based on 4852 article reviews
    exonuclease i enzyme - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Transfection:

    Article Title: Kinase domain insert containing receptor promotor controlled suicide gene system kills human umbilical vein endothelial cells
    Article Snippet: Shutter plasmid pAdtrack, adenoviral backbone plasmid pAdEasy-1 and E. coli BJ5183 were provided by Dr. Belt Vogelstein of Johns Hopkins Oncology Center, Howard Hughes Institute of Medicine. pMD18-T vector was purchased from TaKaRa Biotechnology (Dalian) Co., Ltd. 293 cells and HUVEC cells were obtained from American Type Culture Collection (ATCC). .. All sorts of exonuclease enzyme, T4 DNA ligase, Taq DNA polymerase were purchased from New England Biolabs Co. DMEM, fetal bovine serum (FBS), transfection reagents, Lipofectamine 2000 were products of Gibco Co. Primers of KDR promoter, CD, TK genes were synthesized and sequenced by Sangon Biotechnology (Shanghai) Co., Ltd. ..

    Article Title: Kinase domain insert containing receptor promotor controlled suicide gene system kills human umbilical vein endothelial cells
    Article Snippet: Materials Shutter plasmid pAdtrack, adenoviral backbone plasmid pAdEasy-1 and E. coli BJ5183 were provided by Dr. Belt Vogelstein of Johns Hopkins Oncology Center, Howard Hughes Institute of Medicine. pMD18-T vector was purchased from TaKaRa Biotechnology (Dalian) Co., Ltd. 293 cells and HUVEC cells were obtained from American Type Culture Collection (ATCC). .. All sorts of exonuclease enzyme, T4 DNA ligase, Taq DNA polymerase were purchased from New England Biolabs Co. DMEM, fetal bovine serum (FBS), transfection reagents, Lipofectamine 2000 were products of Gibco Co. Primers of KDR promoter, CD, TK genes were synthesized and sequenced by Sangon Biotechnology (Shanghai) Co., Ltd. Plasmid pAdtrackKDR-CDglyTK construction Primers for polymerase chain reaction (PCR) amplifying KDR promoter gene (including the minimus core of the gene sequences-226-+268) were designed according to the sequences provided by GenBank {"type":"entrez-nucleotide","attrs":{"text":"X89776","term_id":"1155008","term_text":"X89776"}} X89776 . ..

    Synthesized:

    Article Title: Kinase domain insert containing receptor promotor controlled suicide gene system kills human umbilical vein endothelial cells
    Article Snippet: Shutter plasmid pAdtrack, adenoviral backbone plasmid pAdEasy-1 and E. coli BJ5183 were provided by Dr. Belt Vogelstein of Johns Hopkins Oncology Center, Howard Hughes Institute of Medicine. pMD18-T vector was purchased from TaKaRa Biotechnology (Dalian) Co., Ltd. 293 cells and HUVEC cells were obtained from American Type Culture Collection (ATCC). .. All sorts of exonuclease enzyme, T4 DNA ligase, Taq DNA polymerase were purchased from New England Biolabs Co. DMEM, fetal bovine serum (FBS), transfection reagents, Lipofectamine 2000 were products of Gibco Co. Primers of KDR promoter, CD, TK genes were synthesized and sequenced by Sangon Biotechnology (Shanghai) Co., Ltd. ..

    Article Title: Kinase domain insert containing receptor promotor controlled suicide gene system kills human umbilical vein endothelial cells
    Article Snippet: Materials Shutter plasmid pAdtrack, adenoviral backbone plasmid pAdEasy-1 and E. coli BJ5183 were provided by Dr. Belt Vogelstein of Johns Hopkins Oncology Center, Howard Hughes Institute of Medicine. pMD18-T vector was purchased from TaKaRa Biotechnology (Dalian) Co., Ltd. 293 cells and HUVEC cells were obtained from American Type Culture Collection (ATCC). .. All sorts of exonuclease enzyme, T4 DNA ligase, Taq DNA polymerase were purchased from New England Biolabs Co. DMEM, fetal bovine serum (FBS), transfection reagents, Lipofectamine 2000 were products of Gibco Co. Primers of KDR promoter, CD, TK genes were synthesized and sequenced by Sangon Biotechnology (Shanghai) Co., Ltd. Plasmid pAdtrackKDR-CDglyTK construction Primers for polymerase chain reaction (PCR) amplifying KDR promoter gene (including the minimus core of the gene sequences-226-+268) were designed according to the sequences provided by GenBank {"type":"entrez-nucleotide","attrs":{"text":"X89776","term_id":"1155008","term_text":"X89776"}} X89776 . ..

    Purification:

    Article Title: Efficient scar-free knock-ins of several kilobases by engineered CRISPR/Cas endonucleases
    Article Snippet: .. Reaction was performed in 100μl 1xNEB4 buffer (50 mM potassium acetate, 20 mM Tris-acetate, 10 mM magnesium acetate, 1 mM DTT, pH 7.9) + 5 mM fresh DTT, 200 nM labelled oligonucleotide, 5U/100μl exonuclease enzyme (T5E (New England Biolabs M0663S) and T7E (New England Biolabs M0263S), □□exonucleases (T7E and T5E) and purified UL12 we applied UL12 with the same molarity as T7E. ..

    Labeling:

    Article Title: Efficient scar-free knock-ins of several kilobases in plants by engineered CRISPR-Cas endonucleases.
    Article Snippet: Fluorescence assays were performed using the TECAN Spark plate reader and blank 96-well plates. .. Emission wavelength (emission = 520 nm; excitation = 495 nm [width 10 nm each]; Z position = 18 173; gain 50) was monitored over 30 min at 28 C. Reaction was performed in 100 ml 13 NEB4 buffer (50 mM potassium acetate, 20 mM Tris-acetate, 10 mM magnesium acetate, 1 mM DTT [pH 7.9]) + 5 mM fresh DTT, 200 nM labeled oligonucleotide, 5 U/100 ml exonuclease enzyme (T5E [New England Biolabs M0663S] and T7E [New England Biolabs M0263S], l exonuclease). ..

    Plasmid Preparation:

    Article Title: Kinase domain insert containing receptor promotor controlled suicide gene system kills human umbilical vein endothelial cells
    Article Snippet: Materials Shutter plasmid pAdtrack, adenoviral backbone plasmid pAdEasy-1 and E. coli BJ5183 were provided by Dr. Belt Vogelstein of Johns Hopkins Oncology Center, Howard Hughes Institute of Medicine. pMD18-T vector was purchased from TaKaRa Biotechnology (Dalian) Co., Ltd. 293 cells and HUVEC cells were obtained from American Type Culture Collection (ATCC). .. All sorts of exonuclease enzyme, T4 DNA ligase, Taq DNA polymerase were purchased from New England Biolabs Co. DMEM, fetal bovine serum (FBS), transfection reagents, Lipofectamine 2000 were products of Gibco Co. Primers of KDR promoter, CD, TK genes were synthesized and sequenced by Sangon Biotechnology (Shanghai) Co., Ltd. Plasmid pAdtrackKDR-CDglyTK construction Primers for polymerase chain reaction (PCR) amplifying KDR promoter gene (including the minimus core of the gene sequences-226-+268) were designed according to the sequences provided by GenBank {"type":"entrez-nucleotide","attrs":{"text":"X89776","term_id":"1155008","term_text":"X89776"}} X89776 . ..

    Polymerase Chain Reaction:

    Article Title: Kinase domain insert containing receptor promotor controlled suicide gene system kills human umbilical vein endothelial cells
    Article Snippet: Materials Shutter plasmid pAdtrack, adenoviral backbone plasmid pAdEasy-1 and E. coli BJ5183 were provided by Dr. Belt Vogelstein of Johns Hopkins Oncology Center, Howard Hughes Institute of Medicine. pMD18-T vector was purchased from TaKaRa Biotechnology (Dalian) Co., Ltd. 293 cells and HUVEC cells were obtained from American Type Culture Collection (ATCC). .. All sorts of exonuclease enzyme, T4 DNA ligase, Taq DNA polymerase were purchased from New England Biolabs Co. DMEM, fetal bovine serum (FBS), transfection reagents, Lipofectamine 2000 were products of Gibco Co. Primers of KDR promoter, CD, TK genes were synthesized and sequenced by Sangon Biotechnology (Shanghai) Co., Ltd. Plasmid pAdtrackKDR-CDglyTK construction Primers for polymerase chain reaction (PCR) amplifying KDR promoter gene (including the minimus core of the gene sequences-226-+268) were designed according to the sequences provided by GenBank {"type":"entrez-nucleotide","attrs":{"text":"X89776","term_id":"1155008","term_text":"X89776"}} X89776 . ..



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    Preparation of ssPCRP. A , visualization via a 2% agarose gel. Lane M: markers. Lanes 1 and 14: single-stranded amplicon with a final length of 100 bp (80 μM). Lanes 2, 5, 8, and 11: PCR products. Lanes 3, 6, 9, and 12: ssPCRP preparation with lambda exonuclease enzyme from PCR products. Lanes 4, 7, 10, and 13: ssPCRP treatment using exonuclease I enzyme. All PCRs were performed using phosphorylated reverse primers (1 μM). Lanes 2, 3, and 4; 5, 6, and 7; 8, 9, and 10; and 11, 12, and 13 have been done using phosphorothioated, phosphorothioated–phosphorylated, normal, and phosphorylated forward primers, respectively, as indicated in the figure, in a final concentration of 1 μM. B , ssPCRP analysis using 6% denaturing PAGE. Lanes 1 and 2: 32 P-labeled 100-base oligonucleotide (0.1 pmol), intact or treated with lambda exonuclease, respectively. Lanes 3 and 6: PCR with phosphorylated reverse primers (1 μM). Lanes 4 and 7: ssPCRP preparation by lambda exonuclease. Lanes 5 and 8: ssPCRP incubation with exonuclease I enzyme. Lanes 3, 4, and 5; and lanes 6, 7, and 8: PCRs were done by employing normal- 32 P-labeled and phosphorothioated- 32 P-labeled forward primers, respectively, in a final amount of 4 pmol. ssPCRP, single-stranded PCR product.

    Journal: The Journal of Biological Chemistry

    Article Title: Simple in vitro single-stranded linear and circular DNA preparation, functional selection, and validation using phosphor-derived modifications

    doi: 10.1016/j.jbc.2025.110874

    Figure Lengend Snippet: Preparation of ssPCRP. A , visualization via a 2% agarose gel. Lane M: markers. Lanes 1 and 14: single-stranded amplicon with a final length of 100 bp (80 μM). Lanes 2, 5, 8, and 11: PCR products. Lanes 3, 6, 9, and 12: ssPCRP preparation with lambda exonuclease enzyme from PCR products. Lanes 4, 7, 10, and 13: ssPCRP treatment using exonuclease I enzyme. All PCRs were performed using phosphorylated reverse primers (1 μM). Lanes 2, 3, and 4; 5, 6, and 7; 8, 9, and 10; and 11, 12, and 13 have been done using phosphorothioated, phosphorothioated–phosphorylated, normal, and phosphorylated forward primers, respectively, as indicated in the figure, in a final concentration of 1 μM. B , ssPCRP analysis using 6% denaturing PAGE. Lanes 1 and 2: 32 P-labeled 100-base oligonucleotide (0.1 pmol), intact or treated with lambda exonuclease, respectively. Lanes 3 and 6: PCR with phosphorylated reverse primers (1 μM). Lanes 4 and 7: ssPCRP preparation by lambda exonuclease. Lanes 5 and 8: ssPCRP incubation with exonuclease I enzyme. Lanes 3, 4, and 5; and lanes 6, 7, and 8: PCRs were done by employing normal- 32 P-labeled and phosphorothioated- 32 P-labeled forward primers, respectively, in a final amount of 4 pmol. ssPCRP, single-stranded PCR product.

    Article Snippet: For ssPCRP preparation, each 10 μl of double-stranded PCR products were treated with 5 units of lambda exonuclease enzyme (New England Biolabs [NEB]) at 37 °C for 45 min, which is followed by 10 min at 75 °C for enzyme inactivation.

    Techniques: Agarose Gel Electrophoresis, Amplification, Concentration Assay, Labeling, Incubation

    PCR product circularization strategy. A , schematic representation of the template used with specific sequences as PCR primer–binding sites ( red line ) at the extremities and random sequences ( dark blue line ) in the middle . B , PCR using reverse primer ( orange line ) with 5′ phosphorylation modification ( green dot ) and forward primer ( light blue line ) with 5′ phosphorylation modification ( green dot ) and five phosphorothioate bonds ( gray line ) at the far 5′ end of the primer. The “A” illustrates the adenine added by the Taq DNA polymerase at the 3′ end of its product. C , exonuclease reaction by utilizing the lambda exonuclease enzyme ( orange semicircle ) for degradation of the nonphosphorothioated reverse strand. D , single-stranded PCR product (ssPCRP) circularization via complementary strand ( black line ). E , head-to-tail sealing of ssPCRP through ligation reaction using a ligase enzyme ( yellow ellipse ). F , finally, nonligated ssPCRP and the complementary strand are degraded by the action of exonuclease I enzyme ( red semicircle ).

    Journal: The Journal of Biological Chemistry

    Article Title: Simple in vitro single-stranded linear and circular DNA preparation, functional selection, and validation using phosphor-derived modifications

    doi: 10.1016/j.jbc.2025.110874

    Figure Lengend Snippet: PCR product circularization strategy. A , schematic representation of the template used with specific sequences as PCR primer–binding sites ( red line ) at the extremities and random sequences ( dark blue line ) in the middle . B , PCR using reverse primer ( orange line ) with 5′ phosphorylation modification ( green dot ) and forward primer ( light blue line ) with 5′ phosphorylation modification ( green dot ) and five phosphorothioate bonds ( gray line ) at the far 5′ end of the primer. The “A” illustrates the adenine added by the Taq DNA polymerase at the 3′ end of its product. C , exonuclease reaction by utilizing the lambda exonuclease enzyme ( orange semicircle ) for degradation of the nonphosphorothioated reverse strand. D , single-stranded PCR product (ssPCRP) circularization via complementary strand ( black line ). E , head-to-tail sealing of ssPCRP through ligation reaction using a ligase enzyme ( yellow ellipse ). F , finally, nonligated ssPCRP and the complementary strand are degraded by the action of exonuclease I enzyme ( red semicircle ).

    Article Snippet: For ssPCRP preparation, each 10 μl of double-stranded PCR products were treated with 5 units of lambda exonuclease enzyme (New England Biolabs [NEB]) at 37 °C for 45 min, which is followed by 10 min at 75 °C for enzyme inactivation.

    Techniques: Binding Assay, Phospho-proteomics, Modification, Ligation